Determination of the Apoptotic Effect of Raf2 and Nck-Interacting Protein Kinase Inhibitor on Metastatic Canine Mammary Gland Tumor Cells

Objective: Canine mammary gland tumors (CMGTs) are the most common in female dogs and main treatment options used CMGTs surgery caused some complications. Therefore, new needed for CMGTs. Traf2 Nck-interacting serine protein kinase (TNIK) as a transcriptional coregulator of Wnt targeted genes is highly expressed breast cancer development. The inhibition TNIK may be therapeutic target cancers with abnormal WNT signaling. Therefore we aimed to investigate potential effect NCB-0846 on metastatic sarcoma subtype, first time. Materials Methods: cytotoxic apoptotic effects CMGT cells were analyzed by WST‑1, Annexin V, cell cycle, acridine orange (AO) DAPI staining. Results: significantly inhibited viability dose time dependent manner (p<0.05) induced nuclear damage cells. Furthermore, death through accumulation G0/G1 phase. Conclusion: Our findings demonstrated that could potentially anti-cancer agent However, further investigations need performed order elucidate inhibitory activity signaling